ph3 millipore 06-570 antibody  (Millipore)

Journal: iScience

Article Title: p21 , ccng1 , foxo3b , and fbxw7 contribute to p53 -dependent cell cycle arrest

doi: 10.1016/j.isci.2025.112558

Figure Lengend Snippet: Loss of puma , noxa , and p21 provide resistance to p53-mediated induction of apoptosis and partially resistance to p53-mediated cell-cycle arrest (A) Experimental workflow showing how samples were harvested. 29-, 27- and 24-h post fertilization (hpf) wildtype, puma −/− ; noxa −/− , pnp −/− and p53 −/− zebrafish embryos were treated with 30 Gy IR-irradiation and fixed at 1-, 3-, 6-, 9- and 12-h post IR-treatment (hpi, 1hpi, 3hpi and 6hpi panels). (B) Representative images of anti-active Caspase-3 staining on 30-hpf zebrafish embryos for each group. Arrows in WT points out active apoptotic area in head region at 3 and 6 hpi. Scale bar: 500μM. (C) Representative images of phospho-histone H3 (pH3)-stained 30-hpf (1 and 3 hpi) or 36-hpf (12 hpi) zebrafish embryos for each group. Experimental design showing in A and A. Scale bar: 500μM. (D) Quantification of pH3 positive cells in treated and untreated WT, pnp −/− and p53 −/− embryos for each group. Each dot represents an individual. The average number of pH3+ cells (Mean) were indicated in each group. Bars represent mean ± SEM. ∗, p < 0.05. ∗∗∗, p < 0.001.∗∗∗∗, p < 0.0001.

Article Snippet: For pH3 staining, the anti-pH3 primary antibody (Cell Signaling, 9701) was used at 1:200 dilution.

Techniques: Irradiation, Staining

Journal: iScience

Article Title: p21 , ccng1 , foxo3b , and fbxw7 contribute to p53 -dependent cell cycle arrest

doi: 10.1016/j.isci.2025.112558

Figure Lengend Snippet: Loss of p21 partially rescues p53 -dependent mdm2 -null induced cell-cycle arrest (A) The conceptional diagram of mdm2 -null induced embryonic lethality. Loss of mdm2 elevates p53 protein levels to induce downstream targets and effector functions to render the lethality. (B) Representative gross images of 24-hpf mdm2 +/+ , mdm2 −/− ; puma −/− ; noxa −/− ; p21 −/− ( mpnp −/− ) and mdm2 −/− ; p53 −/− embryos. Scale bar: 500μM. (C) pH3-stained mdm2 +/+ , mdm2 −/− ; mpnp −/− embryos at 12-, 16-, 20- and 24-hpf. Scale bar: 200μM. (D) Quantification of pH3 positive cells at 12 hpf (Top panel) and at 24 hpf (bottom panel). Each dot represents an individual. Bars represent mean ± SEM. ∗∗∗, p < 0.001.∗∗∗∗, p < 0.0001. Not statistical significance between mdm2 −/− and mpnp −/− at 24 hpf.

Article Snippet: For pH3 staining, the anti-pH3 primary antibody (Cell Signaling, 9701) was used at 1:200 dilution.

Techniques: Staining

Journal: iScience

Article Title: p21 , ccng1 , foxo3b , and fbxw7 contribute to p53 -dependent cell cycle arrest

doi: 10.1016/j.isci.2025.112558

Figure Lengend Snippet: fbxw7 , foxo3b and ccng1 G0 crispants mitigate p53 -mediated cell-cycle arrest (A) Representative images showing pH3-stained un-injected (control, un-inj) and injected mpnp −/− embryos at 21 hpf. Scale bar: 250μM. (B) Quantification of pH3 positive cells in injected mpnp −/− embryos for 24 GOIs. un-inj (negative control) and the p53 guides-injected ( p53 , positive control). (C) Representative images representing pH3-stained, IR-irradiation treated or untreated, four-guide injected pnp −/− embryos at 30 hpf. Scale bar: 500μM. (D) Quantification of pH3 positive cells in injected pnp −/− embryos for fbxw7 , foxo3b and ccng1 . p21 -inj (negative control) and p53 -inj (positive control). Each dot represents an individual. Bars represent mean ± SEM. ∗, p < 0.05. ∗∗, p < 0.01.∗∗∗, p < 0.001.∗∗∗∗, p < 0.0001.

Article Snippet: For pH3 staining, the anti-pH3 primary antibody (Cell Signaling, 9701) was used at 1:200 dilution.

Techniques: Staining, Injection, Control, Negative Control, Positive Control, Irradiation

Journal: iScience

Article Title: p21 , ccng1 , foxo3b , and fbxw7 contribute to p53 -dependent cell cycle arrest

doi: 10.1016/j.isci.2025.112558

Figure Lengend Snippet:

Article Snippet: For pH3 staining, the anti-pH3 primary antibody (Cell Signaling, 9701) was used at 1:200 dilution.

Techniques: Software, Expressing, Functional Assay

Journal: Cell Regeneration

Article Title: Mongolian medicine Eerdun-Wurile promotes myocardial regeneration by regulating MVDA in zebrafish

doi: 10.1186/s13619-025-00235-z

Figure Lengend Snippet: Eerdun-Wurile (EW) reduces apoptosis of cardiomyocytes and promotes proliferation of cardiomyocytes. Representative images of TUNEL staining in the control, metronidazole (MTZ) ablation, EW treatment groups and EW alone at 48 hpt ( A ), 72 hpt ( C ). White arrows indicate TUNEL positive (TUNEL +) cardiomyocytes (CMs). Statistical analysis of apoptosis cardiomyocyte at 48 ( B ), 72 hpt ( D ). Immunofluorescent analysis of cardiomyocytes proliferation at 48 ( E ), 72 ( G ), and 96 hpt ( I ). White arrows indicate phosphorylated histone H3 positive (pH3 +) cardiomyocytes (CMs). Statistical analysis of cardiomyocytes proliferation at 48 ( F ), 72 ( H ), and 96 hpt ( J ). *, P < 0.05; **, P < 0.01. Scale bar, 20 μm

Article Snippet: Samples were blocked with blocking buffer (10% goat serum in PBS containing 0.5% Triton X-100) for 2 h at 22~25°C and then incubated with a primary antibody against pH3 (1:500; Cat. No.: 3377; Cell Signalling Technology, Boston, USA) and MVD (1:500, Cat.No.

Techniques: TUNEL Assay, Staining, Control

Journal: Cell Regeneration

Article Title: Mongolian medicine Eerdun-Wurile promotes myocardial regeneration by regulating MVDA in zebrafish

doi: 10.1186/s13619-025-00235-z

Figure Lengend Snippet: mvda affects the proliferation of cardiomyocytes. Immunofluorescent analysis of cardiomyocytes proliferation at 48 ( A ), 72 hpt ( C ) in mvda overexpression and knocking down group. White arrows indicate phosphorylated histone H3 positive (pH3 +) cardiomyocytes (CMs). Statistical analysis of cardiomyocytes proliferation at 48 ( B ), 72 hpt ( D ),. *, P < 0.05; **, P < 0.01. Scale bar, 20 μm

Article Snippet: Samples were blocked with blocking buffer (10% goat serum in PBS containing 0.5% Triton X-100) for 2 h at 22~25°C and then incubated with a primary antibody against pH3 (1:500; Cat. No.: 3377; Cell Signalling Technology, Boston, USA) and MVD (1:500, Cat.No.

Techniques: Over Expression